Plus M-10 User Manual

© 2010 Nexcelom Bioscience LLCCellometer Auto M10, CellometerAuto T4 & Cellometer Auto T4Plus User Manual

Getting Started

Getting Started12© 2010 Nexcelom Bioscience LLCGetting Started

13© 2010 Nexcelom Bioscience LLC2 Getting StartedThis section helps you to get started using the Cellometer Auto systems for cell counting.Installati

Getting Started14© 2010 Nexcelom Bioscience LLCInstallation procedureA.Check computer systemThe computer operating system (Windows 2000, XP, Vista, 7

Installation15© 2010 Nexcelom Bioscience LLC3. Click “Install”

Getting Started16© 2010 Nexcelom Bioscience LLC4. Click "Finish"5. The Cellomter Auto Counter icon will appear on your desktop and is read

Installation17© 2010 Nexcelom Bioscience LLC3. Select the option "Yes, this time only"4. Click “Next”5. Select “Install the software automat

Getting Started18© 2010 Nexcelom Bioscience LLC7. Click “Continue Anyway”8. Click “Finish”Note: some computer systems may require USB driver installat

Installation19© 2010 Nexcelom Bioscience LLC2. Enter instrument serial number which is located on the back of the Cellometer.3. You will see a warning

The Cellometer Auto is a high precision imaging instrument. If you have difficulty concerningoperation or installation, please contact Customer Servic

Getting Started20© 2010 Nexcelom Bioscience LLCSet up Cellometer Auto for cell counting2.2This section goes through the complete procedure of counting

Set up Cellometer Auto for cell counting21© 2010 Nexcelom Bioscience LLCHold the loaded chamber in the white area and stay away from the clear optical

Getting Started22© 2010 Nexcelom Bioscience LLCClick "Count" button while the cell image is displayed. ("Display Image" button mu

Software Features

Software Features24© 2010 Nexcelom Bioscience LLCSoftware Features

25© 2010 Nexcelom Bioscience LLC3 Software FeaturesThis section describes software features of Cellometer Auto Counter software. Cellometer software

Software Features26© 2010 Nexcelom Bioscience LLC24) # of live and dead cells: + or - number of live or dead cells for manual adjustment25) Manual Adj

Cellometer software panel27© 2010 Nexcelom Bioscience LLCCellometer software menu items3.2Load Image for DisplayLoad one image from saved Cellometer A

Software Features28© 2010 Nexcelom Bioscience LLCSet Current as DefaultSet Cell Type currently selected in the Set Up box as the Cell type after resta

Cellometer software menu items29© 2010 Nexcelom Bioscience LLC“Counting Options”Count All - count all cells in total areasSpeed Count - stop counting

Note: This equipment has been tested and found to comply with the limits for a Class A digital device,pursuant to part 15 of the FCC Rules. These lim

Software Features30© 2010 Nexcelom Bioscience LLC"Save Options"Set sample ID as cell type: Checking this box will auto input the Sample ID

Cellometer software menu items31© 2010 Nexcelom Bioscience LLCSave raw images / Save counted images: Checking these boxes indicate which type of imag

Software Features32© 2010 Nexcelom Bioscience LLC Show Size DistributionPrint: print cell size histogramSave As: save cell diameter values to a data

Cellometer software menu items33© 2010 Nexcelom Bioscience LLCThis menu item includes the following functions.Help ContentsThe online help consists of

Operation Reference

Operation Reference36© 2010 Nexcelom Bioscience LLCOperation Reference

37© 2010 Nexcelom Bioscience LLC4 Operation ReferenceThis section describes recommended procedures to perform some routine operations with Cellometer

Operation Reference38© 2010 Nexcelom Bioscience LLC8. Cell Type -> Edit Cell Type to modify cell type parameters. 9. Recount the same images using

Multiple sample histogram39© 2010 Nexcelom Bioscience LLCMultiple sample histogram4.9Use the following steps for this function.1. Analysis -> Cell

Tutorial

Tutorial42© 2010 Nexcelom Bioscience LLCTutorial

43© 2010 Nexcelom Bioscience LLC5 TutorialThis section includes several tutorials to illustrate how to perform some routine functions with Cellometer

Tutorial44© 2010 Nexcelom Bioscience LLC5. Enter in dilution factor6. Count cells7. Output displays "Live Cell Concentration" (cells/ml), an

Test viability45© 2010 Nexcelom Bioscience LLCSet up a new cell type5.3Method 1: Import from Cell LibraryImport pre-existing cell type parameters from

Tutorial46© 2010 Nexcelom Bioscience LLCAdjust cell size parameters5.4For each cell type, "Cell Diameter Max." and "Cell Diameter Min.&

Adjust cell size parameters47© 2010 Nexcelom Bioscience LLCAdjust dead cell parameters5.5Default settings of “Dead Cell Parameters” provide fastest co

Tutorial48© 2010 Nexcelom Bioscience LLCDead cells with a brighter centerDead cells of this type have small bright centers, as shown in the following

Adjust dead cell parameters49© 2010 Nexcelom Bioscience LLCAdjust decluster parameters for clumpy cells5.6For decluster function, there are two parame

5© 2010 Nexcelom Bioscience LLCTable of ContentsPart IIntroduction and Description8...

Tutorial50© 2010 Nexcelom Bioscience LLCTurn on and off declustering for small cells5.7Examples of small cells are mouse splenocytes, lymphocytes, thy

Turn on and off declustering for small cells51© 2010 Nexcelom Bioscience LLCHowever, some samples contain clumpy cells, as shown in the following figu

Tutorial52© 2010 Nexcelom Bioscience LLCCalculator #1: Desired cell concentrationInput: Target Concentration Click: Apply Change Display: Instruction

Sample adjustment after cell couting53© 2010 Nexcelom Bioscience LLCCell size analysis5.9Sample AdjustmentMeasured Concentration: cell concentration m

Tutorial54© 2010 Nexcelom Bioscience LLCPrint: print cell size histogramSave As: save cell diameter values to a data fileOpen Diameter File: open an e

Appendix

Appendix56© 2010 Nexcelom Bioscience LLCAppendix

57© 2010 Nexcelom Bioscience LLC6 AppendixSpectifications6.1Cellometer Auto is an imaging instrument that acquires cell data from multiple locations

Appendix58© 2010 Nexcelom Bioscience LLCa. On your PC, go to Start->Control Panelb. Double click “System”The operating System must be Windows 2000,

Computer check procedure59© 2010 Nexcelom Bioscience LLC\Cell Samples – Contains sample imagesFiles:AutoT4-HEK293-A.bmpAutoT4-Hela-A.bmpAutoT4-HT29_bl

Cellometer Auto M10, Cellometer Auto T4 & Cellometer Auto T4 Plus User Manual6© 2010 Nexcelom Bioscience LLC...

Appendix60© 2010 Nexcelom Bioscience LLCUSB memory stick installsIt is often the case that installing from a USB memory stick will result in a failure

Customer ServiceNexcelom Bioscience LLC360 Merrimack StreetBuilding 9Lawrence, MA 01843, USAPhone: 978-327-5340Fax: 978-327-5341Email: support@

Introduction and Description

Introduction and Description8© 2010 Nexcelom Bioscience LLCIntroduction and Description

9© 2010 Nexcelom Bioscience LLC1 Introduction and DescriptionThe basic principle of the Cellometer® automatic cell counter is imaging cytometry. It i