© 2010 Nexcelom Bioscience LLCCellometer Auto M10, CellometerAuto T4 & Cellometer Auto T4Plus User Manual
Getting Started
Getting Started12© 2010 Nexcelom Bioscience LLCGetting Started
13© 2010 Nexcelom Bioscience LLC2 Getting StartedThis section helps you to get started using the Cellometer Auto systems for cell counting.Installati
Getting Started14© 2010 Nexcelom Bioscience LLCInstallation procedureA.Check computer systemThe computer operating system (Windows 2000, XP, Vista, 7
Installation15© 2010 Nexcelom Bioscience LLC3. Click “Install”
Getting Started16© 2010 Nexcelom Bioscience LLC4. Click "Finish"5. The Cellomter Auto Counter icon will appear on your desktop and is read
Installation17© 2010 Nexcelom Bioscience LLC3. Select the option "Yes, this time only"4. Click “Next”5. Select “Install the software automat
Getting Started18© 2010 Nexcelom Bioscience LLC7. Click “Continue Anyway”8. Click “Finish”Note: some computer systems may require USB driver installat
Installation19© 2010 Nexcelom Bioscience LLC2. Enter instrument serial number which is located on the back of the Cellometer.3. You will see a warning
The Cellometer Auto is a high precision imaging instrument. If you have difficulty concerningoperation or installation, please contact Customer Servic
Getting Started20© 2010 Nexcelom Bioscience LLCSet up Cellometer Auto for cell counting2.2This section goes through the complete procedure of counting
Set up Cellometer Auto for cell counting21© 2010 Nexcelom Bioscience LLCHold the loaded chamber in the white area and stay away from the clear optical
Getting Started22© 2010 Nexcelom Bioscience LLCClick "Count" button while the cell image is displayed. ("Display Image" button mu
Software Features
Software Features24© 2010 Nexcelom Bioscience LLCSoftware Features
25© 2010 Nexcelom Bioscience LLC3 Software FeaturesThis section describes software features of Cellometer Auto Counter software. Cellometer software
Software Features26© 2010 Nexcelom Bioscience LLC24) # of live and dead cells: + or - number of live or dead cells for manual adjustment25) Manual Adj
Cellometer software panel27© 2010 Nexcelom Bioscience LLCCellometer software menu items3.2Load Image for DisplayLoad one image from saved Cellometer A
Software Features28© 2010 Nexcelom Bioscience LLCSet Current as DefaultSet Cell Type currently selected in the Set Up box as the Cell type after resta
Cellometer software menu items29© 2010 Nexcelom Bioscience LLC“Counting Options”Count All - count all cells in total areasSpeed Count - stop counting
Note: This equipment has been tested and found to comply with the limits for a Class A digital device,pursuant to part 15 of the FCC Rules. These lim
Software Features30© 2010 Nexcelom Bioscience LLC"Save Options"Set sample ID as cell type: Checking this box will auto input the Sample ID
Cellometer software menu items31© 2010 Nexcelom Bioscience LLCSave raw images / Save counted images: Checking these boxes indicate which type of imag
Software Features32© 2010 Nexcelom Bioscience LLC Show Size DistributionPrint: print cell size histogramSave As: save cell diameter values to a data
Cellometer software menu items33© 2010 Nexcelom Bioscience LLCThis menu item includes the following functions.Help ContentsThe online help consists of
Operation Reference
Operation Reference36© 2010 Nexcelom Bioscience LLCOperation Reference
37© 2010 Nexcelom Bioscience LLC4 Operation ReferenceThis section describes recommended procedures to perform some routine operations with Cellometer
Operation Reference38© 2010 Nexcelom Bioscience LLC8. Cell Type -> Edit Cell Type to modify cell type parameters. 9. Recount the same images using
Multiple sample histogram39© 2010 Nexcelom Bioscience LLCMultiple sample histogram4.9Use the following steps for this function.1. Analysis -> Cell
Tutorial
Tutorial42© 2010 Nexcelom Bioscience LLCTutorial
43© 2010 Nexcelom Bioscience LLC5 TutorialThis section includes several tutorials to illustrate how to perform some routine functions with Cellometer
Tutorial44© 2010 Nexcelom Bioscience LLC5. Enter in dilution factor6. Count cells7. Output displays "Live Cell Concentration" (cells/ml), an
Test viability45© 2010 Nexcelom Bioscience LLCSet up a new cell type5.3Method 1: Import from Cell LibraryImport pre-existing cell type parameters from
Tutorial46© 2010 Nexcelom Bioscience LLCAdjust cell size parameters5.4For each cell type, "Cell Diameter Max." and "Cell Diameter Min.&
Adjust cell size parameters47© 2010 Nexcelom Bioscience LLCAdjust dead cell parameters5.5Default settings of “Dead Cell Parameters” provide fastest co
Tutorial48© 2010 Nexcelom Bioscience LLCDead cells with a brighter centerDead cells of this type have small bright centers, as shown in the following
Adjust dead cell parameters49© 2010 Nexcelom Bioscience LLCAdjust decluster parameters for clumpy cells5.6For decluster function, there are two parame
5© 2010 Nexcelom Bioscience LLCTable of ContentsPart IIntroduction and Description8...
Tutorial50© 2010 Nexcelom Bioscience LLCTurn on and off declustering for small cells5.7Examples of small cells are mouse splenocytes, lymphocytes, thy
Turn on and off declustering for small cells51© 2010 Nexcelom Bioscience LLCHowever, some samples contain clumpy cells, as shown in the following figu
Tutorial52© 2010 Nexcelom Bioscience LLCCalculator #1: Desired cell concentrationInput: Target Concentration Click: Apply Change Display: Instruction
Sample adjustment after cell couting53© 2010 Nexcelom Bioscience LLCCell size analysis5.9Sample AdjustmentMeasured Concentration: cell concentration m
Tutorial54© 2010 Nexcelom Bioscience LLCPrint: print cell size histogramSave As: save cell diameter values to a data fileOpen Diameter File: open an e
Appendix
Appendix56© 2010 Nexcelom Bioscience LLCAppendix
57© 2010 Nexcelom Bioscience LLC6 AppendixSpectifications6.1Cellometer Auto is an imaging instrument that acquires cell data from multiple locations
Appendix58© 2010 Nexcelom Bioscience LLCa. On your PC, go to Start->Control Panelb. Double click “System”The operating System must be Windows 2000,
Computer check procedure59© 2010 Nexcelom Bioscience LLC\Cell Samples – Contains sample imagesFiles:AutoT4-HEK293-A.bmpAutoT4-Hela-A.bmpAutoT4-HT29_bl
Cellometer Auto M10, Cellometer Auto T4 & Cellometer Auto T4 Plus User Manual6© 2010 Nexcelom Bioscience LLC...
Appendix60© 2010 Nexcelom Bioscience LLCUSB memory stick installsIt is often the case that installing from a USB memory stick will result in a failure
Customer ServiceNexcelom Bioscience LLC360 Merrimack StreetBuilding 9Lawrence, MA 01843, USAPhone: 978-327-5340Fax: 978-327-5341Email: support@
Introduction and Description
Introduction and Description8© 2010 Nexcelom Bioscience LLCIntroduction and Description
9© 2010 Nexcelom Bioscience LLC1 Introduction and DescriptionThe basic principle of the Cellometer® automatic cell counter is imaging cytometry. It i
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